Reducing off-target events in CRISPR genome editing applications with a high-fidelity Cas9 nuclease
1:00:39
YouTubeIntegrated DNA Technologies (IDT)
Reducing off-target events in CRISPR genome editing applications with a high-fidelity Cas9 nuclease
The CRISPR-Cas9 system demonstrates unparalleled genome editing efficiency in a broad range of species and cell types, but it suffers from concerns related to target specificity. Modified guide RNAs and mutant Cas9 proteins have been developed to reduce off-target editing but, in many cases, the alterations also significantly reduce on-target ...
5.9K viewsAug 16, 2017
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